EndoFree Maxi Plasmid Kit

Mo te purenga o te endotoxin-kore whakawhiti ira grade plasmid DNA.

Ko te EndoFree Maxi Plasmid Kit kei runga i te hangarau membrane silica hei tango i te DNA plasmid kore-endotoxin. Ko tenei kete e whakarato ana i tetahi ahurei tangohanga endotoxin ahurei hei whakaputa i te DNA plasmid-kounga tiketike mo nga tono whakararo. I hangaia hei wehe i te DNA plasmid mo te tino ma o te 100-200 ml o te ahurea kitakita, ka taea te tuku ki te 1.5 mg te whakarahi i te DNA plasmid.

Ngeru. No. Rahinga Peke
4992194 10 preps

Taipitopito Hua

Tauira Whakamatau

Tohu Hua

Āhuahira

■ Hua tere me te nui: 200 μg-1.5 mg hua DNA plasmid i roto i te 1 haora me te rahi o te hanganga super-koromeke.
■ Maama-nui: Ko te maamaa-nui o te DNA plasmid ka riro mai i te punaha miihini ahurei me te Panui Tīwae CP6.
■ Tino pai o te whakawhitinga: He pai mo nga whakamatautau whakawhitinga o te nuinga o nga raina o te rauropi.
■ Te whanui o nga tono: He pai mo te aukati i te whākōkī o te whākōkī, te whakarerekētanga, te raupapa, te microinjection, te noho puku o te ira me ngā whakamātautau whakawhiti.

Hua Plasmid

Plasmid Yield

Ka taea te whakarite i nga hua katoa mo te ODM / OEM. Mo nga taipitopito,pāwhiritia koa Ratonga Whakaritea (ODM / OEM)


  • Tōmua:
  • Panuku:

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    Experimental Example Ko te tangohanga tiketike / iti o te tangohanga plasmid
    Ko te iti-kape plasmid pBR322, i tangohia mai i te 200 ml ahurea kitakita ma te whakamahi i te EndoFree Maxi Plasmid Kit, i tukuna ki te 1 ml Buffer TB, me te kukume o te 0.6 μg / μl. Ko te pBS plasmid kape-nui, i tangohia mai i te 100 ml ahurea kitakita ma te whakamahi i te EndoFree Maxi Plasmid Kit, i tukuna ki te 1 ml Buffer TB, me te kukume 1.2 μg / μl.
    MIV: TIANGEN DNA Tohu IV
    pBR322: 2 μl, pBS: 2 μl
    Experimental Example Ko te pEGFP i whiwhi me te EndoFree Maxi Plasmid Kit i wehe ke atu ki te raina o te rauropi endotoxin-kore aro 293T me nga raina o te rekoata-endotoxin-Huh7 mai i a TIANGEN TIANfect. Ko te whakaputa i te GFP i kitea i roto i nga haora 24 i muri i te whakawhiti.
    Tuhia to korero ki konei ka tuku mai ki a maatau