DNA Bisulfite Conversion Kit Featured Image

Kete Huringa DNA Bisulfite

Ko te hurihanga me te purea ka taea te whakaoti i roto i te 2 haora, me te reanga faafariu ki te 99%.

Ko te kete hurihuri Bisulfite DNA he mea whakawhanakehia mo te hurihanga bisulfite DNA i roto i te rangahau methylation DNA. Ko te maimoatanga o nga tauira DNA e whakamahi ana i tenei kete ka taea te whakaoti i roto i nga haora 2, a ko te rereketanga o te cytosine unmethylated ki te uracil kei runga ake i te 99%. Ko tenei kete ka tango i tetahi waahanga motuhake o te kaihoko tiaki DNA, e whakamana ana i te kounga me te whakaora o te DNA i muri i te hurihanga. Hei taapiri, kei te whakamahia e tenei kete te tikanga mo te tango i te roopu waikawa sulphurous-a-runga, kia ngawari ake ai te mahinga whakahaere.

Ngeru. No.	Rahinga Peke
4992447	50 preps

Tukuna imeera ki a maatau

Taipitopito Hua

Tauira Whakamatau

Tohu Hua

Āhuahira

■ Maamaa me te tere: Ka taea te whakaoti i te waahanga hurihanga me te horoi i roto i nga haora 2, me nga whakaritenga iti mo nga taonga taputapu me nga taputapu, e pai ana mo nga whare rangahau i nga taumata katoa.
■ Te reanga nui: Ko te rereketanga o te cytosine kaore i hangaia ki te uracil i roto i te tauira DNA neke atu i te 99%.
■ Te mohio nui: Ka taea e tenei kete te tukatuka i nga tauira DNA kia rite ki te 500 pg tae atu ki te 2.5 μg.

Whakatakotoranga

Momo: Tahuringa Bisulfite
Tauira DNA: 500 pg-2.5 μg
Te reanga faafariu: > 99%
Te wa mahi: ~ 2 haora
Nga tono: Ko nga tauira DNA e tukatukahia ana e tenei kete e pai ana mo te methylation motuhake PCR / qPCR, te raupapa me te microarray.

Ka taea te whakarite i nga hua katoa mo te ODM / OEM. Mo nga taipitopito,pāwhiritia koa Ratonga Whakaritea (ODM / OEM)

Tōmua: TIANSeq mRNA Kete Hopu

Panuku: Methylation-specipc PCR (MSP) kete

Ripanga 1: Te tere whakawhiti o te "C" ki te "U"
Ma te whakamahi i te ira a-tangata hei taonga whakamatautau, ko te tere whakaora o te tauira ka eke ki te 65%, ko te tauwehenga o te "C" me te "U" kaore e neke atu i te 99%.

Table 1: Conversion rate of unmethylated “C” to “U” Using human genome as experimental material, the recovery rate of the sample can reach 65%, and the ratio of unmethylated “C” to “U” can reach more than 99%.